On April 1, 2025, and late March, reports highlighted Donald Trump's preparation to unveil reciprocal tariffs, potentially as early as Tuesday night, causing global market concerns. Some Republican senators warned of a potential global trade war, while Trump insisted action was needed due to the US being “ripped off”. These tariffs also threaten the US craft beer industry and are expected to impact the UK, with Downing Street preparing for announcements and reserving the right to respond. The Trump administration is also discussing financial aid for farmers potentially impacted by retaliatory tariffs, with a potential aid package similar to the 2018 trade war with China.

In late March 2025, Greenland’s Prime Minister Jens-Frederik Nielsen rejected President Trump’s assertion that the United States would annex Greenland. Trump told NBC News on March 30, 2025, that the United States would "get Greenland," expressing confidence in the annexation. An American delegation, led by Vice President JD Vance, visited Greenland on March 29, 2025, which was viewed by some Greenlandic officials as an aggressive escalation of Trump's threats to seize the territory.

On April 1, 2025, Donald Trump’s executive order seeking to overhaul US elections faced its first legal challenges as the Democratic National Committee and a pair of nonprofits filed two separate lawsuits calling it unconstitutional. Also on this day, Democrats are suing President Trump over his executive order on elections.

On April 1, 2025, a federal judge in San Francisco paused the Trump administration's plan to end temporary legal protections for hundreds of thousands of Venezuelans, scheduled for April 7, 2025.

On April 1, 2025, a federal judge ruled that Alabama could not prosecute doctors and reproductive health organizations for helping patients travel out of state to obtain abortions.

On April 1, 2025, it was reported that the Trump administration is withholding tens of millions of dollars for Planned Parenthood chapters across the US in an attempt to force the clinics to change their operations.

On April 1, 2025, internal documents revealed that Trump’s Department of Energy plans to cut funding from hundreds of clean energy projects, disproportionately affecting California and other Democratic states.

On March 31, 2025, news emerged that Dr. Peter Marks, the government’s top vaccine expert at FDA, resigned over the weekend, citing the administration’s “unprecedented assault on scientific truth”.

On March 31, 2025, reports indicated that the Trump administration has sought to cut more than 1,100 scientists as it dissolves the Environmental Protection Agency’s (EPA) office of research and development. The Trump administration is also seeking to cut as much as one-fifth of the workforce from the National Oceanic and Atmospheric Administration (Noaa).

On April 1, 2025, the Trump administration announced a review of federal contracts and grants at Harvard University over allegations of antisemitism.

On April 1, 2025, Trump took aim at ticket scalping in a new executive order signed today, which directs the Department of Justice and the FTC to crack down on ticket resellers who price-gouge.

On March 31, 2025, over 1,900 members of the National Academies of Sciences, Engineering and Medicine signed an open letter warning about the Trump administration’s attacks on science.

Health Secretary Robert F. Kennedy Jr. announced the administration would seek to cut approximately 20,000 jobs from the Department of Health and Human Services (HHS).

On April 1, 2025, voters in Wisconsin and Florida headed to the polls in elections seen as a test of Donald Trump’s popularity and Elon Musk’s political clout. The Wisconsin Supreme Court race became a referendum on Elon Musk's influence, who invested over $20 million backing Brad Schimel. In Florida, special elections to replace House members Michael Waltz and Matt Gaetz, who joined Trump's cabinet, are tighter than expected, with Trump endorsing Jimmy Patronis to replace Gaetz and Randy Fine to replace Waltz.

Elon Musk's Super Pac has spent over $20 million in the Wisconsin Supreme Court race, where Trump has endorsed Brad Schimel.

On April 1, 2025, Lt. Gen. Dan Caine attended a Senate hearing regarding his nomination to become the chairman of the Joint Chiefs of Staff. General Caine’s loyalty to the Constitution and his reputation for adhering to ethical standards are major reasons the Senate Armed Services Committee is likely to give him a largely friendly reception.

On April 1, 2025, Senator John Thune commented on Donald Trump's remarks about running for a third term, suggesting Trump was "probably messing with you".

On April 1, 2025, PBS Chief Executive Paula Kerger, and Katherine Maher, who heads NPR, testified before Congress, led by Georgia Republican Rep. Marjorie Taylor Greene, to defend against charges of liberal bias and advocate for public broadcasting.

On April 1, 2025, voters in Louisiana rejected four proposed constitutional amendments backed by Republican Gov. Jeff Landry, which aimed to overhaul tax codes and increase penalties for juvenile offenders.

On April 1, 2025, film director Oliver Stone testified at a US House of Representatives hearing regarding the newly released documents related to the 1963 assassination of John F Kennedy, released under President Donald Trump's direction. Also on this day, Cory Booker delivered an all-night speech on the Senate floor criticizing President Trump's actions.